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Plant Mutation Breeding And Biotechnology Pdf

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Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding

Not a MyNAP member yet? Register for a free account to start saving and receiving special member only perks. This chapter explores the likelihood of unintended effects from diverse methods of genetic modification of plants and animals see Operational Definitions in Chapter 1. Specifically, it discusses unexpected outcomes of breeding methods used to develop a food crop or strain and unexpected or unintended effects recorded in the scientific literature. It also includes analyses of methods intentionally used for modifying food sources and comparing the likelihood of unintended changes resulting from the use of genetic engineering versus other methods of genetic modification discussed in Chapter 2. Novel gene combinations arising from the genetic manipulation of existing genes through conventional breeding techniques may introduce unintended and unexpected effects.

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New Breeding Techniques NBTs include several new technologies for introduction of new variation into crop plants for plant breeding, in particular the methods that aim to make targeted mutagenesis at specific sites in the plant genome NBT mutagenesis. The decision was based in a context of conventional plant breeding using mutagenesis of crop plants by physical or chemical treatments. These plants are explicitly exempted from the EU GMO legislation, based on the long-termed use of mutagenesis. Thus, by both strategies mutations in any gene across the genome can be obtained at a rather high speed. However, the differences between the strategies are 1 the precision of the exact site of mutation in a target gene, and 2 the number of off-target mutations affecting other genes than the target gene. Both aspects favour the NBT methods, which provide more precision and fewer off-target mutations.

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Nielen S. Mutagen effects in the first generation after seed treatment: biological effects of mutation treatments. Manual on Mutation Breeding — Third edition. Rome, Italy. Free download of whole book:. Click to access ien. It also provides a comprehensive overview and guidelines for new high-throughput screening methods — both phenotypic and genotypic — that are currently available to enable the detection of rare and valuable mutant traits and reviews techniques for increasing the efficiency of crop mutation breeding.

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University of Nairobi, P. Plant Breeding and Genetics. Plant breeding is defined as identifying and selecting desirable traits in plants and combining these into one individual plant. Sharma Director of Research Dr.

Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding.

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Q Y Shu-Plant mutation breeding and biotechnology-CABI (2012)

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